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Department of Quantitative Biomedicine

The Bodenmiller group develops an end-to-end multiplexed image processing and analysis workflow

See Windhager et al., Nature Protocols

In this work, the Bodenmiller group presents an end-to-end workflow for multiplexed tissue image processing and analysis that integrates previously developed computational tools to enable image segmentation, feature extraction and spatially resolved single-cell analysis in a user-friendly and customizable way. This protocol can be implemented by researchers with basic bioinformatics training, and the analysis of the provided dataset can be completed within 5–6 h.

An extended version is available at

Fig. 1: Overview of the multiplexed tissue image analysis workflow. © The Authors, 2023