Heterogeneity of signalling networks is at the basis of many biological processes, such as cell differentiation and drug resistance. Recent developments in multiplexed single-cell measurement technologies enabled evaluation of this heterogeneity. This review categorizes single-cell signaling network profiling approaches by their methodology, coverage, and application, and discusses the pros and cons of each technology. Furthermore, computational tools for network characterization using single-cell data are described. Finally, potential confounding factors that need to be considered in single-cell signaling network analyses are discussed.
See Lun & Bodenmiller, Molecular & Cellular Proteomics