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Department of Quantitative Biomedicine

The Bodenmiller group develops multielement Z-tag imaging by X-ray fluorescence microscopy

Multielement Z-tag imaging by X-ray fluorescence microscopy for next-generation multiplex imaging

See Strotton et al., Nature Methods

Whole-organism to atomic-level imaging is possible with tissue-penetrant, picometer-wavelength X-rays. To enable highly multiplexed X-ray imaging, the Bodenmiller group developed multielement Z-tag X-ray fluorescence (MEZ-XRF) that can operate at kHz speeds when combined with signal amplification by exchange reaction (SABER)-amplified Z-tag reagents. Parallel imaging of 20 Z-tag or SABER Z-tag reagents at subcellular resolution was demonstrated in cell lines and multiple human tissues. The unique multiscale, nondestructive nature of MEZ-XRF, combined with SABER Z-tags for high sensitivity or enhanced speed, enables highly multiplexed bioimaging across biological scales.

Fig 1a. Stratton 2023
https://uzh.ch/de.html
Fig 1a. MEZ-XRF involves (1) staining of biological samples with Z-tagged affinity reagents, (2) raster scanning of a focused X-ray beam over the stained sample and collecting emission spectra for each pixel, (3) deconvoluting spectra into multichannel images and (4) analyzing images. © The Authors, 2023